1) 2X CTAB extraction buffer 6 ml + beta-mercaptoethanol 30 ul at temperature at 65 ํC
2) sample curcuma 1 g
3) Incubate at 65 ํC for 1 hour <back up and down every 15 min>
4) chloroform:isoamyle (24:1) 6 ml centrifuge at 6,000 rpm for 25 min
5) transfer supernatant to new tube 600 ul, add 1/10 3M NaOAC (pH5.2) 60 ul, add 1 time 100% ethanol 600 ul (back up and down tube). Incubate at -20 ํC for 1 hour —> stop
6) centrifuge at 12,000 rpm 4ํC for 10 min
7) wash pellet with 70% ethanal 1 ml for 1 min centrifuge at 12,000 rpm
8) dry pellet 10 min
9)dissolved with D.I. water 30 ul


